Graphene oxide in a composite with silver nanoparticles reduces the fibroblast and endothelial cell cytotoxicity of an antibacterial nanoplatform

Mateusz Wierzbicki , Sławomir Jaworski , Ewa Sawosz , Anna Jung , Grzegorz Gielerak , Henryk Jaremek , Witold Łojkowski , Bartosz Woźniak , Leszek Stobiński , Artur Małolepszy , André Chwalibog

Abstract

Antibacterial surfaces coated with nanomaterials, including silver nanoparticles, are considered effective alternative antimicrobial agents that can be used instead of antibiotics and chemical agents. However, reports of the potential toxicity of these materials raise questions about the safety of their use in biomedical applications. The objective of this research was to reduce the human cell cytotoxicity of silver nanoparticle-coated polyurethane foils by complexing silver nanoparticles with graphene oxide. The antimicrobial activity of nanoplatforms coated with silver nanoparticles, graphene oxide and the composite of silver nanoparticles and graphene oxide was assessed with Salmonella enteritidis. Cytotoxicity was analysed by an analysis of the viability and morphology of human fibroblasts, human umbilical vein endothelial cells (HUVECs) and chicken embryo chorioallantoic membrane. Additionally, the synthesis level of inflammatory proteins was examined for fibroblasts cultured on different nanoplatforms. The nanoplatform coated with the silver nanoparticles and graphene oxide composite showed strongest antibacterial properties, although nanoplatforms coated with only silver nanoparticles or graphene oxide also resulted in decreased S. enteritidis growth. Furthermore, a nanoplatform coated with silver nanoparticles and graphene oxide composite showed limited immunological stimulation and significantly reduced cytotoxicity towards fibroblasts, HUVECs and chicken embryo chorioallantoic membrane in comparison to the nanoplatform coated only with silver nanoparticles, due to the higher stability of the nanomaterials in the nanocomposite.
Author Mateusz Wierzbicki
Mateusz Wierzbicki,,
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, Sławomir Jaworski
Sławomir Jaworski,,
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, Ewa Sawosz
Ewa Sawosz,,
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, Anna Jung - Military Institute of Medicine
Anna Jung,,
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, Grzegorz Gielerak
Grzegorz Gielerak,,
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, Henryk Jaremek
Henryk Jaremek,,
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, Witold Łojkowski - Instytut Wysokich Ciśnień Polskiej Akademii Nauk (Instytut Wysokich Ciśnień Polskiej Akademii Nauk), MNiSW [80]
Witold Łojkowski,,
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, Bartosz Woźniak - Instytut Wysokich Ciśnień Polskiej Akademii Nauk (Instytut Wysokich Ciśnień Polskiej Akademii Nauk)
Bartosz Woźniak,,
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, Leszek Stobiński (FCPE / GL)
Leszek Stobiński,,
- WUT Grafen Laboratory
, Artur Małolepszy (FCPE / DSP)
Artur Małolepszy,,
- Department of Separation Processes
et al.`
Journal seriesNanoscale Research Letters, ISSN 1556-276X, [1931-7573], (N/A 100 pkt)
Issue year2019
Vol14
No1
Pages1-11
Publication size in sheets0.5
Article number320
Keywords in Englishsilver nanoparticles, graphene oxide, antibacterial surface, toxicity, fibroblasts, endothelial cells
ASJC Classification2500 General Materials Science; 3104 Condensed Matter Physics
DOIDOI:10.1186/s11671-019-3166-9
URL https://link-1springer-1com-1000096sc03af.eczyt.bg.pw.edu.pl/article/10.1186/s11671-019-3166-9
Languageen angielski
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Wierzbicki M. (i in.) - Graphene oxide in...pdf 3.01 MB
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 09-01-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 0.874; WoS Impact Factor: 2018 = 3.159 (2) - 2018=3.411 (5)
Citation count*
Additional fields
UwagiThis work was supported by an internal grant of the Military Institute ofMedicine no 419 and by the Polish National Research Council grant NCN2016/23/D/NZ9/01401
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