Lack of Maf1 enhances pyruvate kinase activity and fermentative metabolism while influencing lipid homeostasis in Saccharomyces cerevisiae

Jolanta Mierzejewska , Karolina Chreptowicz

Abstract

The Maf1 protein is a general negative repressor of RNA polymerase III, which is conserved in eukaryotes from yeast to humans. Herein, we show the yeast maf1D mutant increases pyruvate kinase activity, the key enzyme in glycolysis and an important player in switching between fermentative and oxidative metabolism. We observed enhanced ethanol production and elevated lipid content in the maf1D strain grown on glucose. However, after shifting to a non-fermentable carbon source, the opposite effect was observed, and the mutant cells accumulated smaller lipid droplets. Thus, it has been concluded that the Maf1 protein is essential for regulation of glucose metabolism and lipid homeostasis.
Author Jolanta Mierzejewska ZTiBSL
Jolanta Mierzejewska,,
- Department Of Drug Technology And Biotechnology
, Karolina Chreptowicz ZTiBSL
Karolina Chreptowicz,,
- Department Of Drug Technology And Biotechnology
Journal seriesFEBS Letters, ISSN 0014-5793
Issue year2016
Vol590
No1
Pages93-100
Publication size in sheets0.5
Keywords in Englishpyruvate kinase, MAF1, pol III, fermentation, lipid droplets, yeast
DOIDOI:10.1002/1873-3468.12033
URL https://www.ncbi.nlm.nih.gov/pubmed/26787463
Languageen angielski
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Score (nominal)30
ScoreMinisterial score = 30.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.623 (2) - 2016=3.424 (5)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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