Lack of Maf1 enhances pyruvate kinase activity and fermentative metabolism while influencing lipid homeostasis in Saccharomyces cerevisiae
Jolanta Mierzejewska , Karolina Chreptowicz
AbstractThe Maf1 protein is a general negative repressor of RNA polymerase III, which is conserved in eukaryotes from yeast to humans. Herein, we show the yeast maf1D mutant increases pyruvate kinase activity, the key enzyme in glycolysis and an important player in switching between fermentative and oxidative metabolism. We observed enhanced ethanol production and elevated lipid content in the maf1D strain grown on glucose. However, after shifting to a non-fermentable carbon source, the opposite effect was observed, and the mutant cells accumulated smaller lipid droplets. Thus, it has been concluded that the Maf1 protein is essential for regulation of glucose metabolism and lipid homeostasis.
|Journal series||FEBS Letters, ISSN 0014-5793|
|Publication size in sheets||0.5|
|Keywords in English||pyruvate kinase, MAF1, pol III, fermentation, lipid droplets, yeast|
|ASJC Classification||; ; ; ; ;|
|Score|| = 30.0, 17-08-2020, ArticleFromJournal|
= 30.0, 17-08-2020, ArticleFromJournal
|Publication indicators||= 7; = 7; : 2016 = 0.894; : 2016 = 3.623 (2) - 2016=3.424 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.