Lack of Maf1 enhances pyruvate kinase activity and fermentative metabolism while influencing lipid homeostasis in Saccharomyces cerevisiae

Jolanta Mierzejewska , Karolina Chreptowicz


The Maf1 protein is a general negative repressor of RNA polymerase III, which is conserved in eukaryotes from yeast to humans. Herein, we show the yeast maf1D mutant increases pyruvate kinase activity, the key enzyme in glycolysis and an important player in switching between fermentative and oxidative metabolism. We observed enhanced ethanol production and elevated lipid content in the maf1D strain grown on glucose. However, after shifting to a non-fermentable carbon source, the opposite effect was observed, and the mutant cells accumulated smaller lipid droplets. Thus, it has been concluded that the Maf1 protein is essential for regulation of glucose metabolism and lipid homeostasis.
Author Jolanta Mierzejewska (FC / IBC / DDTB)
Jolanta Mierzejewska,,
- Department Of Drug Technology And Biotechnology
, Karolina Chreptowicz (FC / IBC / DDTB)
Karolina Chreptowicz,,
- Department Of Drug Technology And Biotechnology
Journal seriesFEBS Letters, ISSN 0014-5793
Issue year2016
Publication size in sheets0.5
Keywords in Englishpyruvate kinase, MAF1, pol III, fermentation, lipid droplets, yeast
ASJC Classification1307 Cell Biology; 1311 Genetics; 1312 Molecular Biology; 1303 Biochemistry; 1315 Structural Biology; 1304 Biophysics
Languageen angielski
wdpb_publikacje_pliki_plik_publikacja_2697_org.pdf 510.22 KB
Score (nominal)30
Score sourcejournalList
ScoreMinisterial score = 30.0, 17-08-2020, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 17-08-2020, ArticleFromJournal
Publication indicators Scopus Citations = 7; WoS Citations = 7; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 0.894; WoS Impact Factor: 2016 = 3.623 (2) - 2016=3.424 (5)
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