Athrombogenic hydrogel coatings for medical devices – Examination of biological properties

Beata Aleksandra Butruk-Raszeja , Ilona Łojszczyk , Tomasz Ciach , Magdalena Kościelniak-Ziemniak , Karolina Janiczak , Roman Kustosz , Małgorzata Gonsior

Abstract

In the article the authors present hydrogel coatings prepared from polyvinylpyrrolidone (PVP) macromolecules, which are chemically bonded to polyurethane (PU) substrate. The coating is designed to improve the surface hemocompatibility of blood-contacting medical devices. The coating was characterized in terms of physical properties (swelling ratio, hydrogel density, surface morphology, coating thickness, coating durability). In order to examine surface hemocompatibility, the materials were contacted with whole human blood under arterial flow simulated conditions followed by calculation of platelet consumption and the number of platelet aggregates. Samples were also contacted with platelet-poor plasma; the number of surface-adsorbed fibrinogen molecules was measured using ELISA assay. Finally, the inflammatory reaction after implantation was assessed, using New Zealand rabbits. The designed coating is characterized by high water content and excellent durability in aqueous environment – over a 35-day period, no significant changes in coating thickness were observed. Experiments with blood proved twice the reduction in adsorption of serum-derived fibrinogen together with a moderate reduction in the number of platelet aggregates formed during the contact of the material with blood. The analysis of an inflammatory reaction after the implantation confirmed high biocompatibility of the fabricated materials – studies have shown no toxic effects of the implanted material on the surrounding animal tissues.
Author Beata Aleksandra Butruk-Raszeja (FCPE / DBBE)
Beata Aleksandra Butruk-Raszeja,,
- Department of Biotechnology and Bioprocess Engineering
, Ilona Łojszczyk (FCPE / DBBE)
Ilona Łojszczyk,,
- Department of Biotechnology and Bioprocess Engineering
, Tomasz Ciach (FCPE / DBBE)
Tomasz Ciach,,
- Department of Biotechnology and Bioprocess Engineering
, Magdalena Kościelniak-Ziemniak - [Foundation for Cardiac Surgery Development]
Magdalena Kościelniak-Ziemniak,,
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, Karolina Janiczak - [Foundation for Cardiac Surgery Development]
Karolina Janiczak,,
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, Roman Kustosz - [Foundation for Cardiac Surgery Development]
Roman Kustosz,,
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, Małgorzata Gonsior - [Foundation for Cardiac Surgery Development]
Małgorzata Gonsior,,
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Journal seriesColloids and Surfaces B-Biointerfaces, ISSN 0927-7765, (A 35 pkt)
Issue year2015
Vol130
Pages192-198
Publication size in sheets0.5
Keywords in Englishpolyurethane, surface modification, blood compatibility, platelet fibrinogen hydrogel
ASJC Classification1505 Colloid and Surface Chemistry; 1606 Physical and Theoretical Chemistry; 3110 Surfaces and Interfaces; 2700 General Medicine; 1305 Biotechnology
DOIDOI:10.1016/j.colsurfb.2015.04.008
URL http://ac.els-cdn.com/S0927776515002155/1-s2.0-S0927776515002155-main.pdf?_tid=687b7854-2098-11e5-be5e-00000aacb35f&acdnat=1435827646_091186b28a4969bd925f4249b25ae03c
Languageen angielski
File
Butruk-Raszeja B. A. (i in.) - Athrombogenic hydrogel coatings....pdf 1.93 MB
Score (nominal)35
Score sourcejournalList
ScoreMinisterial score = 35.0, 30-01-2020, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 30-01-2020, ArticleFromJournal
Publication indicators Scopus Citations = 8; WoS Citations = 4; Scopus SNIP (Source Normalised Impact per Paper): 2015 = 1.232; WoS Impact Factor: 2015 = 3.902 (2) - 2015=4.269 (5)
Citation count*15 (2020-02-15)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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