Athrombogenic hydrogel coatings for medical devices – Examination of biological properties
Beata Aleksandra Butruk-Raszeja , Ilona Łojszczyk , Tomasz Ciach , Magdalena Kościelniak-Ziemniak , Karolina Janiczak , Roman Kustosz , Małgorzata Gonsior
AbstractIn the article the authors present hydrogel coatings prepared from polyvinylpyrrolidone (PVP) macromolecules, which are chemically bonded to polyurethane (PU) substrate. The coating is designed to improve the surface hemocompatibility of blood-contacting medical devices. The coating was characterized in terms of physical properties (swelling ratio, hydrogel density, surface morphology, coating thickness, coating durability). In order to examine surface hemocompatibility, the materials were contacted with whole human blood under arterial flow simulated conditions followed by calculation of platelet consumption and the number of platelet aggregates. Samples were also contacted with platelet-poor plasma; the number of surface-adsorbed fibrinogen molecules was measured using ELISA assay. Finally, the inflammatory reaction after implantation was assessed, using New Zealand rabbits. The designed coating is characterized by high water content and excellent durability in aqueous environment – over a 35-day period, no significant changes in coating thickness were observed. Experiments with blood proved twice the reduction in adsorption of serum-derived fibrinogen together with a moderate reduction in the number of platelet aggregates formed during the contact of the material with blood. The analysis of an inflammatory reaction after the implantation confirmed high biocompatibility of the fabricated materials – studies have shown no toxic effects of the implanted material on the surrounding animal tissues.
|Journal series||Colloids and Surfaces B-Biointerfaces, ISSN 0927-7765, (A 35 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||polyurethane, surface modification, blood compatibility, platelet fibrinogen hydrogel|
|ASJC Classification||; ; ; ;|
|Score|| = 35.0, 30-01-2020, ArticleFromJournal|
= 35.0, 30-01-2020, ArticleFromJournal
|Publication indicators||= 8; = 4; : 2015 = 1.232; : 2015 = 3.902 (2) - 2015=4.269 (5)|
|Citation count*||15 (2020-02-15)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.