The influence of surfactants and hydrolyzed proteins on keratinocytes viability and elasticity

Tomasz Kobiela , Karolina Leleń-Kamińska , Michał Stepulak , Małgorzata Lekka , Magdalena Malejczyk , Jacek Arct , Sławomir Majewski

Abstract

Background/purpose The knowledge how surfactants and hydrolyzed proteins influence the elastic properties of living epidermal keratinocytes is sparse. We demonstrate that the stiffness of cells measured by atomic force microscope (AFM) can be correlated with viability test. Methods and materials The effects of sodium lauryl sulphate (SLS) and hydrolyzed collagen (HK) of molecular weight 9 kDa were examined with respect to human keratinocytes viability and elasticity. MTT assay was applied to determine the survival fraction of keratinocytes treated with SLS and HK solutions of various molar ratios. The AFM measurements of the keratinocytes stiffness were carried out immediately after the exposure of cells to the SLS and HK, respectively. Results The increase of the SLS concentration resulted in the decrease of cells proliferation and this effect was inhibited by addition of HK. The strongest inhibition was observed for the SLS:HK molar ratio equals to 2:1. AFM study shows decrease in the cell stiffness for cells treated with SLS. Fluorescence microscopy reveals remodeling of actin filaments of SLS-treated cells. SLS:HK mixture treatment results in mechanical stiffness close to untreated cells. Conclusion These results provide possible correlations between mechanical properties and viability of keratinocytes when the chemical stress occurs.
Author Tomasz Kobiela (FC / IBC / DDTB)
Tomasz Kobiela,,
- Department Of Drug Technology And Biotechnology
, Karolina Leleń-Kamińska - [Academy of Cosmetics and Health Care]
Karolina Leleń-Kamińska,,
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, Michał Stepulak - [Academy of Cosmetics and Health Care]
Michał Stepulak,,
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, Małgorzata Lekka - [Henryk Niewodniczanski Institute of Nuclear Physics of the Polish Academy of Sciences]
Małgorzata Lekka,,
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, Magdalena Malejczyk - [Medical University of Warsaw]
Magdalena Malejczyk,,
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, Jacek Arct
Jacek Arct,,
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, Sławomir Majewski - [Academy of Cosmetics and Health Care]
Sławomir Majewski,,
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Journal seriesSkin Research and Technology, ISSN 0909-752X
Issue year2013
Vol19
No1
Pagese200-e208
Publication size in sheets0.5
Keywords in Englishatomic force microscopy ; cytoskeleton ; hydrolyzed collagen ; keratinocyte stiffness ; sodium lauryl sulphate
ASJC Classification2708 Dermatology
DOIDOI:10.1111/j.1600-0846.2012.00628.x
URL http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0846.2012.00628.x/abstract
Languageen angielski
File
skinresearchandtechnology_2013_19_e200.pdf 309.05 KB
Score (nominal)30
Score sourcejournalList
ScoreMinisterial score = 30.0, 29-02-2020, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 29-02-2020, ArticleFromJournal
Publication indicators Scopus Citations = 10; WoS Citations = 8; GS Citations = 4.0; Scopus SNIP (Source Normalised Impact per Paper): 2014 = 0.881; WoS Impact Factor: 2013 = 1.536 (2) - 2013=1.919 (5)
Citation count*4 (2015-03-09)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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