Elemental speciation analysis in biochemistry by electrospray mass spectrometry

Hubert Chassaigne , Véronique Vacchina , Ryszard Łobiński


The recent advances and trends in the development and applications of electrospray mass spectrometry (ESI-MS) to speciation analysis are highlighted. The use of the different operational modes of ESI-MS: elemental mode (source collision-induced dissociation (SCID)), molecular mode (MS of protonated molecule ions) and tandem MS mode (product ion scan of the collision-induced dissociation (CID) products of the protonated molecule ion), is discussed. Advantages and limitations of ESI-MS for signal identification in high performance liquid chromatography (HPLC)–ICP-MS and as a free-standing tool for species-selective analysis are highlighted. The discussion is illustrated with a number of examples related to the trace element speciation in biochemistry, viz. selenium in yeast, arsenic in seaweeds, cobalamins in pharmaceutical preparations, and Cd complexes with phytochelatins and metallothioneins in plant and animal tissues
Author Hubert Chassaigne
Hubert Chassaigne,,
, Véronique Vacchina
Véronique Vacchina,,
, Ryszard Łobiński (FC / CAC)
Ryszard Łobiński,,
- Chair Of Analytical Chemistry
Journal seriesTrac-Trends in Analytical Chemistry, ISSN 0165-9936
Issue year2000
Keywords in EnglishArsenic, Cobalamin, Electrospray mass spectrometry, Metallothionein, Phytochelatin, Selenium, Speciation
ASJC Classification1607 Spectroscopy; 1602 Analytical Chemistry
URL http://www.sciencedirect.com/science/article/pii/S0165993699002174
Score (nominal)50
Publication indicators WoS Citations = 54; Scopus SNIP (Source Normalised Impact per Paper): 2000 = 1.42; WoS Impact Factor: 2006 = 5.068 (2) - 2007=5.894 (5)
Citation count*66 (2015-02-27)
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