Collagen immobilization on 316L stainless steel surface with cathodic deposition of calcium phosphate

Agata Roguska , Sachiko Hiromoto , Akiko Yamamoto , Michał Jerzy Woźniak , Marcin Pisarek , Małgorzata Lewandowska

Abstract

Collagen fibril/(calcium phosphate and carbonate) composite coatings on 316L stainless steel were developed with a cathodic deposition technique. The response of SaOS-2 osteoblast-like cells to the collagen/calcium salt-coated 316L steel was investigated. The collagen fibrils were self-assembled on the 316L steel surface and immobilized by their partial incorporation into a calcium salt layer electrodeposited cathodically in Hanks’ solution. The amount of calcium salt depended on the applied cathodic potential. The mineralization of collagen fibrils was observed. The collagen coverage localized and the composition of calcium salts varied on the same specimen. Such non-uniform surfaces affected the cell response. The observed outlines of cell bodies and nuclei on the thin collagen coating were clearer than those on the thick collagen coating in most cases. The collagen coating did not significantly influence the mean viability of cells on the whole specimen surface. Interestingly, the alkaline phosphatase activity per cell on the collagen/calcium salt-coated specimens was higher than that on the as-received specimen. It was revealed that cathodic deposition is an effective technique to immobilize collagen fibrils on a 316L steel surface.
Author Agata Roguska
Agata Roguska,,
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, Sachiko Hiromoto
Sachiko Hiromoto,,
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, Akiko Yamamoto
Akiko Yamamoto,,
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, Michał Jerzy Woźniak
Michał Jerzy Woźniak,,
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, Marcin Pisarek
Marcin Pisarek,,
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, Małgorzata Lewandowska ZPM
Małgorzata Lewandowska,,
- Division of Materials Design
Journal seriesApplied Surface Science, ISSN 0169-4332
Issue year2011
Vol257
No11
Pages5037-5045
Keywords in English316L stainless steel, Calcium carbonate, Calcium phosphate, collagen, Electrochemical treatment, SaOS-2 osteoblast-like cells
DOIDOI:10.1016/j.apsusc.2011.01.018
URL http://www.sciencedirect.com/science/article/pii/S0169433211000262
Score (nominal)30
Publication indicators WoS Impact Factor [Impact Factor WoS]: 2011 = 2.103 (2) - 2011=2.032 (5)
Citation count*10 (2015-05-02)
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