Identification of dimethylarsinoyl-riboside derivatives in seaweed by pneumatically assisted electrospray tandem mass spectrometry
Shona McSheehy , Marek Marcinek , Hubert Chassaigne , Joanna Szpunar
AbstractPneumatically-assisted electrospray tandem mass spectrometry (ES MS/MS) was proposed as a technique for the identification of dimethylarsinoyl-riboside derivatives (arsenosugars) in seaweed (Laminaria). Conditions for the acquisition of MS and MS/MS spectra were optimized. A size-exclusion HPLC (SE HPLC) step was developed for the purification of algal extracts prior to ES MS. The arsenosugar fraction was found to elute prior to the majority of other arsenic compounds. The identity of the compounds expected to be arsenosugar compounds was confirmed by the collision induced dissociation (CID) of the relevant protonated molecule ions. An independent confirmation of the identity of analytes was obtained by two-dimensional (size-exclusion–anion exchange) HPLC–ICP MS with signal identification by spiking with the appropriate arsenosugar standards.
|Journal series||Analytica Chimica Acta, ISSN 0003-2670|
|Keywords in English||algae, Arsenosugars, ES MS/MS, HPLC–ICP MS, Speciation|
|ASJC Classification||; ; ;|
|Publication indicators||= 53; = 64.0; : 2000 = 1.158; : 2006 = 2.894 (2) - 2007=3.348 (5)|
|Citation count*||64 (2015-03-26)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.