Casein kinase II-mediated phosphorylation of general repressor Maf1 triggers RNA polymerase III activation

Damian Graczyk , Janusz Dębski , Grażyna Muszyńska , Maria Bretner , Olivier Lefebvre , Magdalena Rakowska-Boguta

Abstract

Maf1 protein is a global negative regulator of RNA polymerase (Pol) III transcription conserved from yeast to man. We report that phosphorylation of Maf1 by casein kinase II (CK2), a highly evolutionarily conserved eukaryotic kinase, is required for efficient Pol III transcription. Both recombinant human and yeast CK2 were able to phosphorylate purified human or yeast Maf1, indicating that Maf1 can be a direct substrate of CK2. Upon transfer of Saccharomyces cerevisiae from repressive to favorable growth conditions, CK2 activity is required for the release of Maf1 from Pol III bound to a tRNA gene and for subsequent activation of tRNA transcription. In a yeast strain lacking Maf1, CK2 inhibition showed no effect on tRNA synthesis, confirming that CK2 activates Pol III via Maf1. Additionally, CK2 was found to associate with tRNA genes, and this association is enhanced in absence of Maf1, especially under repressive conditions. These results corroborate the previously reported TFIIIB–CK2 interaction and indicate an important role of CK2-mediated Maf1 phosphorylation in triggering Pol III activation.
Author Damian Graczyk
Damian Graczyk,,
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, Janusz Dębski
Janusz Dębski,,
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, Grażyna Muszyńska
Grażyna Muszyńska,,
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, Maria Bretner ZTiBSL
Maria Bretner,,
- Department Of Drug Technology And Biotechnology
, Olivier Lefebvre
Olivier Lefebvre,,
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, Magdalena Rakowska-Boguta ZTiBSL
Magdalena Rakowska-Boguta,,
- Department Of Drug Technology And Biotechnology
Journal seriesProceedings of the National Academy of Sciences, ISSN 0027-8424, 1091-6490
Issue year2011
Vol108
No12
Pages4926-4931
Keywords in Englishcasein kinase II regulation, RNA polymerase III regulation, transfer RNA
DOIDOI:10.1073/pnas.1010010108
URL http://www.pnas.org/content/108/12/4926
Languageen angielski
Score (nominal)0
Citation count*24 (2015-03-11)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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