A sensitive and versatile method for characterization of protein-mediated transformations of quantum dots

Magdalena Matczuk , Joanna Legat , Andrei R. Timerbaev , Maciej Jarosz

Abstract

We report the development and application of an analytical system consisting of capillary electrophoresis (CE) interfaced with inductively coupled plasma mass spectrometry (ICP-MS) for sensitive and high-resolution characterization of quantum dots (QDs) interacting with serum proteins. Separation resolution between the intact CdSeS/ZnS QDs and their protein conjugates was optimized by varying the type and concentration of background electrolyte, applied voltage, and sample loading. Special attention was paid to the CE system compatibility with physiological conditions, avoiding aggregation effects, and analyte recovery. Optimization trials allowed for acquiring satisfactory stability of migration times (within 6.0% between different days), peak area precision of 5.2–8.0%, capillary recoveries in the range of 90–96%, and a lower limit of detection of 7.5 × 10−9 mol L−1 Cd. With the developed method distinct metal-specific profiles were obtained for the QDs in combination with individual serum proteins, their mixtures, and in human serum. Particularly, it was found that albumin binding to the particle surface is completed after 1 h, without noticeable disruption of the core–shell integrity. The transferrin adsorption is accompanied by the removal of the ZnS shell, resulting in evolving two different metal–protein conjugated forms. On the other hand, proteinization in real-serum environment occurs without binding to major transport proteins, the QDs also lose their the shell (the higher the dose the longer is the time they stay unbroken). The concomitant changes in migration behavior can be attributed to interactions with serum proteins other than albumin and transferrin. Speciation information provided by CE-ICP-MS may shed light on the mechanism of QD delivery to the target regions of the body.
Author Magdalena Matczuk KChA
Magdalena Matczuk,,
- Chair Of Analytical Chemistry
, Joanna Legat KChA
Joanna Legat,,
- Chair Of Analytical Chemistry
, Andrei R. Timerbaev
Andrei R. Timerbaev,,
-
, Maciej Jarosz KChA
Maciej Jarosz,,
- Chair Of Analytical Chemistry
Journal seriesAnalyst, ISSN 0003-2654
Issue year2016
Vol141
No8
Pages2574-2580
Publication size in sheets0.5
DOIDOI:10.1039/c6an00276e
URL http://pubs.rsc.org/en/Content/ArticleLanding/2016/AN/C6AN00276E#!divAbstract
Languageen angielski
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wdpb_publikacje_pliki_plik_publikacja_2832_org.pdf (file archived - login or check accessibility on faculty) wdpb_publikacje_pliki_plik_publikacja_2832_org.pdf 832.06 KB
Score (nominal)40
ScoreMinisterial score = 40.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.885 (2) - 2016=3.865 (5)
Citation count*0 (2016-09-05)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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