Interactions of benzo[a]pyrene and diesel exhaust particulate matter with the lung surfactant system
Tomasz Robert Sosnowski , Michał Koliński , Leon Gradoń
AbstractOne of the reasons for cellular changes in the lung tissue exposed to the diesel exhaust composed of soot particles with adsorbed volatile organic molecules is the reduction of the clearance rate in the pulmonary region of the respiratory system. The interaction of the fractal-like particles and organic substances with a surfactant monolayer limits its dynamic activity. The surface properties of Survanta, a purified extract of bovine lung surfactant (LS), which interacted with carbon particles (200 nm aggregates) and benzo[a]pyrene (BaP), molecules were measured with the oscillating bubble technique. The results showed a significant lowering of the dynamics of the surfactant monolayer compared to the control case (no exposure). Additional measurements of surface pressure during the monotonic compression of the air–water interface containing the major LS phospholipid, dipalmitoylphosphatidylcholine (DPPC), showed that the presence of BaP molecules in the system influenced its stability. The experimental results were supplemented with a theoretical molecular dynamics model of the interaction between BaP and DPPC molecules. The simulation results indicated the insertion of BaP molecules into the lipid layer, which explained the measured effects.
|Journal series||Annals of Occupational Hygiene, ISSN 0003-4878, (A 25 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||aerosols, benzo[a]pyrene, clearance, diesel exhaust, molecular dynamics, phospholipids, retention, surfactants|
|Publication indicators||= 23; = 21; : 2014 = 1.556; : 2011 = 1.949 (2) - 2011=2.084 (5)|
|Citation count*||29 (2020-01-17)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.