Optimisation of a direct plating method for the detection and enumeration of Alicyclobacillus acidoterrestris spores

Marek Henczka , Małgorzata Djas , Katarzyna Filipek

Abstract

A direct plating method for the detection and enumeration of Alicyclobacillus acidoterrestris spores has been optimised. The results of the application of four types of growth media (BAT agar, YSG agar, K agar and SK agar) regarding the recovery and enumeration of A. acidoterrestris spores were compared. The influence of the type of applied growth medium, heat shock conditions, incubation temperature, incubation time, plating technique and the presence of apple juice in the sample on the accuracy of the detection and enumeration of A. acidoterrestris spores was investigated. Among the investigated media, YSG agar was the most sensitive medium, and its application resulted in the highest recovery of A. acidoterrestris spores, while K agar and BAT agar were the least suitable media. The effect of the heat shock time on the recovery of spores was negligible. When there was a low concentration of spores in a sample, the membrane filtration method was superior to the spread plating method. The obtained results show that heat shock carried out at 80 °C for 10 min and plating samples in combination with membrane filtration on YSG agar, followed by incubation at 46 °C for 3 days provided the optimal conditions for the detection and enumeration of A. acidoterrestris spores. Application of the presented method allows highly efficient, fast and sensitive identification and enumeration of A. acidoterrestris spores in food products. This methodology will be useful for the fruit juice industry for identifying products contaminated with A. acidoterrestris spores, and its practical application may prevent economic losses for manufacturers.
Author Marek Henczka (FCPE / DCRED)
Marek Henczka,,
- Department of Chemical Reactor Engineering and Dynamics
, Małgorzata Djas (FCPE / DCRED)
Małgorzata Djas,,
- Department of Chemical Reactor Engineering and Dynamics
, Katarzyna Filipek - [Warsaw University of Technology (PW)]
Katarzyna Filipek,,
-
- Politechnika Warszawska
Journal seriesJournal of Microbiological Methods, ISSN 0167-7012
Issue year2013
Vol92
No1
Pages1-8
Publication size in sheets0.5
Keywords in Englishalicyclobacillus acidoterrestris, detection method, growth medium, membrane filtration, spores
ASJC Classification2726 Microbiology (medical); 1312 Molecular Biology; 2404 Microbiology
DOIDOI:10.1016/j.mimet.2012.10.007
URL http://www.sciencedirect.com/science/article/pii/S0167701212003405
Languageen angielski
File
Henczka M., Djas M. - Optimisation of a....pdf 843.41 KB
Score (nominal)25
Score sourcejournalList
ScoreMinisterial score = 25.0, 24-04-2020, ArticleFromJournal
Ministerial score (2013-2016) = 25.0, 24-04-2020, ArticleFromJournal
Publication indicators WoS Citations = 6; Scopus Citations = 6; GS Citations = 6.0; Scopus SNIP (Source Normalised Impact per Paper): 2013 = 1.012; WoS Impact Factor: 2013 = 2.096 (2) - 2013=2.348 (5)
Citation count*6 (2016-09-15)
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back
Confirmation
Are you sure?