Bioprocess development in single-use systems for heterotrophic marine microalgae

Friederike Hillig , Steffi Annemüller , Magdalena Chmielewska , Maciej Pilarek , Stefan Junne , Peter Neubauer


The utilization of single-use systems is more and more common in upstream cultivation in biotechnological processes. The advantages of single-use bioreactors for the cultivation of the heterotrophic microalgae Crypthecodinium cohnii serves as an example for the feasibility of polymer based orbital shaken systems for shear-sensitive microorganisms with a high oxygen demand. Experiments in the shake flask scale indicate the advantage of tubes like the TubeSpin Bioreactor 600 in comparison to standard Erlenmeyer flasks. In the screening stage, performed in orbitally shaken deepwell plates, the growth performance is significantly improved by the application of perflourodecalin for the modulation of the gas transfer. The use of orbitally shaken systems from the deepwell plate format over the TubeSpin towards orbitally shaken bioreactors provides a reliable strategy for the consistent bioprocess development and scale up of shear-sensitive aerobic microorganisms. The consistent scalability of the process up to 200 L is envisaged.
Author Friederike Hillig - [Technical University of Berlin]
Friederike Hillig,,
, Steffi Annemüller - [Technical University of Berlin]
Steffi Annemüller,,
, Magdalena Chmielewska (FCPE)
Magdalena Chmielewska,,
- Faculty of Chemical and Process Engineering
, Maciej Pilarek (FCPE / DBBE)
Maciej Pilarek,,
- Department of Biotechnology and Bioprocess Engineering
, Stefan Junne - [Technical University of Berlin]
Stefan Junne,,
, Peter Neubauer - [Technical University of Berlin (TU Berlin)]
Peter Neubauer,,
- Technische Universität Berlin
Journal seriesChemie Ingenieur Technik, ISSN 0009-286X
Issue year2013
Publication size in sheets0.5
Keywords in Englishcrypthecodinium cohnii, orbital shaking, perfluorodecalin, polyunsaturated fatty acid, scale-up
ASJC Classification2209 Industrial and Manufacturing Engineering; 1500 General Chemical Engineering; 1600 General Chemistry
Languageen angielski
Hillig F. (i in.) - Bioprocess Development in....pdf 555.69 KB
Score (nominal)20
Score sourcejournalList
ScoreMinisterial score = 15.0, 06-08-2020, ArticleFromJournal
Ministerial score (2013-2016) = 20.0, 06-08-2020, ArticleFromJournal
Publication indicators WoS Citations = 13; Scopus Citations = 12; GS Citations = 22.0; Scopus SNIP (Source Normalised Impact per Paper): 2013 = 0.493; WoS Impact Factor: 2013 = 0.661 (2) - 2013=0.498 (5)
Citation count*22 (2020-09-14)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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