Maf1 Protein, Repressor of RNA Polymerase III, Indirectly Affects tRNA Processing

Iwona Karkusiewicz , Tomasz W. Turowski , Damian Graczyk , Joanna Towpik , Nripesh Dhungel , Anita K. Hopper , Magdalena Rakowska-Boguta

Abstract

Maf1 is negative regulator of RNA polymerase III in yeast. We observed high levels of both primary transcript and end-matured, intron-containing pre-tRNAs in the maf1Δ strain. This pre-tRNA accumulation could be overcome by transcription inhibition, arguing against a direct role of Maf1 in tRNA maturation and suggesting saturation of processing machinery by the increased amounts of primary transcripts. Saturation of the tRNA exportin, Los1, is one reason why end-matured intron-containing pre-tRNAs accumulate in maf1Δ cells. However, it is likely possible that other components of the processing pathway are also limiting when tRNA transcription is increased. According to our model, Maf1-mediated transcription control and nuclear export by Los1 are two major stages of tRNA biosynthesis that are regulated by environmental conditions in a coordinated manner.
Author Iwona Karkusiewicz (FC / IBC / DM)
Iwona Karkusiewicz,,
- Department Of Microbioanalytics
, Tomasz W. Turowski (FC / IBC / DDTB)
Tomasz W. Turowski,,
- Department Of Drug Technology And Biotechnology
, Damian Graczyk
Damian Graczyk,,
-
, Joanna Towpik
Joanna Towpik,,
-
, Nripesh Dhungel
Nripesh Dhungel,,
-
, Anita K. Hopper
Anita K. Hopper,,
-
, Magdalena Rakowska-Boguta (FC / IBC / DDTB)
Magdalena Rakowska-Boguta,,
- Department Of Drug Technology And Biotechnology
Journal seriesJournal of Biological Chemistry, ISSN 0021-9258, 1083-351X, (B 0 pkt)
Issue year2011
Vol286
No45
Pages39478-39488
Keywords in EnglishMaf1, RNA Polymerase III, RNA Transport, Transcription Repressor, Transfer RNA (tRNA), tRNA Nuclear Export, Yeast
DOIDOI:10.1074/jbc.M111.253310
URL http://www.jbc.org/content/286/45/39478
Languageen angielski
Score (nominal)0
Citation count*17 (2015-03-11)
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