Capillary-HPLC with tandem mass spectrometry in analysis of alkaloid dyestuffs - a new approach

Damian Dąbrowski , Katarzyna Lech , Maciej Jarosz


Development of the identification method of alkaloid compounds in Amur cork tree as well as not examined so far Oregon grape and European Barberry shrubs are presented. The novel approach to separation of alkaloids was applied and the capillary-high-performance liquid chromatography (capillary-HPLC) system was used, which has never previously been reported for alkaloid-based dyestuffs analysis. Its optimization was conducted with three different stationary phases (unmodified octadecylsilane-bonded silica, octadecylsilane modified with polar groups and silica-bonded pentaflourophenyls) as well as with different solvent buffers. Detection of the isolated compounds was carried out using diode-array detector (DAD) and tandem mass spectrometer with electrospray ionization (ESI MS/MS). The working parameters of ESI were optimized, whereas the multiple reactions monitoring (MRM) parameters of MS/MS detection were chosen based on the product ion spectra of the quasi-molecular ions. Calibration curve of berberine has been estimated (y = 1712091x + 4785.03 with the correlation coefficient 0.9999). Limit of detection and limit of quantification were calculated to be 3.2 and 9.7 ng/mL, respectively. Numerous alkaloids (i.e., berberine, jatrorrhizine and magnoflorine, as well as phellodendrine, menisperine and berbamine) were identified in the extracts from alkaloid plants and silk and wool fibers dyed with these dyestuffs, among them their markers.
Author Damian Dąbrowski (FC / CAC)
Damian Dąbrowski,,
- Chair Of Analytical Chemistry
, Katarzyna Lech (FC / CAC)
Katarzyna Lech,,
- Chair Of Analytical Chemistry
, Maciej Jarosz (FC / CAC)
Maciej Jarosz,,
- Chair Of Analytical Chemistry
Journal seriesElectrophoresis, ISSN 0173-0835, (A 30 pkt)
Issue year2018
Publication size in sheets0.3
Keywords in English berberine, capillary-HPLC-ESI MS/MS, fragmentation pathways, natural alkaloid dyestuffs
ASJC Classification1308 Clinical Biochemistry; 1303 Biochemistry
Languageen angielski
wdpb_publikacje_pliki_plik_publikacja_3451_org.pdf 545.59 KB
Score (nominal)30
ScoreMinisterial score = 30.0, 23-09-2019, ArticleFromJournal
Publication indicators WoS Citations = 1; Scopus Citations = 1; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 0.703; WoS Impact Factor: 2017 = 2.569 (2) - 2017=2.298 (5)
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