Screening of yeasts for the production of 2-phenylethanol (rose aroma) in organic waste-based media

Karolina Chreptowicz , Magdalena Sternicka , Patrycja Kowalska , Jolanta Mierzejewska

Abstract

In this study, we isolated 28 yeast strains from samples of plant material and fermented food and evaluated the possibility of efficient production of 2-phenylethanol (2-PE) in the organic waste-based media supplemented with l-phenylalanine (l-Phe). We used whey, a by-product from milk processing, as a base for media, and either glucose or three by-products from sugar beet processing as a fermentable carbon source. Ten newly isolated yeast strains were capable of producing over 2 g l-1 2-PE through the l-Phe biotransformation in a batch mode in standard medium. Among them, we selected eight strains producing 2-PE in a range of 1·17-3·28 g l-1 in 72 h batch cultures in shaking flasks in whey-based media. The strains were assigned to five species of Meyerozyma caribbica, Metschnikowia chrysoperlae, Meyerozyma guilliermondii, Pichia fermentans and Saccharomyces cerevisiae. While S. cerevisiae is known to be a promising producer of 2-PE, the four latter species are poorly studied on this application. Results presented here are better than other reported values for batch cultures of unmodified yeast strains. Therefore, it seems that whey and by-products from sugar beet processing might be a good feedstock for 2-PE bioproduction.
Author Karolina Chreptowicz (FC / IBC / DDTB)
Karolina Chreptowicz,,
- Department Of Drug Technology And Biotechnology
, Magdalena Sternicka
Magdalena Sternicka,,
-
, Patrycja Kowalska
Patrycja Kowalska,,
-
, Jolanta Mierzejewska (FC / IBC / DDTB)
Jolanta Mierzejewska,,
- Department Of Drug Technology And Biotechnology
Journal seriesLetters in Applied Microbiology, ISSN 0266-8254, (A 20 pkt)
Issue year2018
Vol66
No2
Pages153-160
Publication size in sheets0.5
Keywords in English2-phenylethanol; bioproduct; biotransformation; sugar beet; whey; yeasts
DOIDOI:10.1111/lam.12835
URL https://www.ncbi.nlm.nih.gov/pubmed/29224193
Languageen angielski
File
lam.12835.pdf 263.79 KB
Score (nominal)25
ScoreMinisterial score = 20.0, 23-07-2018, ArticleFromJournal
Ministerial score (2013-2016) = 25.0, 23-07-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 1.575 (2) - 2016=1.803 (5)
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