Comparison of fixed and living biological cells parameters investigated with digital holographic microscope
Authors:
- Patryk Kamil Nienałtowski,
- Maria Baczewska,
- Małgorzata Kujawińska
Abstract
The statistical analysis and comparison of biophysical parameters of living and fixed, mouse embryonic fibroblasts cells are presented. The parameters are calculated based on phase measurements performed by means of a digital, holographic microscope. The phases are retrieved from off-axis, image plane holograms, followed by custom image segmentation and statistical analysis of cells’ surface, phase volume and dry mass. The results indicated statistically significant differences between fixed and living cell parameters, which is an important message for setting methodology for further diagnosis based on quantitative phase (label-free) analysis.
- Record ID
- WUT0cda2d018b7e440f9be8c05e8c07aaa4
- Author
- Journal series
- Photonics Letters of Poland, ISSN 2080-2242
- Issue year
- 2020
- Vol
- 12
- No
- 1
- Pages
- 13-15
- Publication size in sheets
- 0.30
- ASJC Classification
- DOI
- DOI:10.4302/plp.v12i1.971 Opening in a new tab
- Language
- (en) English
- Score (nominal)
- 40
- Score source
- journalList
- Score
- = 40.0, 26-05-2022, ArticleFromJournal
- Publication indicators
- = 0; = 0; : 2018 = 0.348
- Uniform Resource Identifier
- https://repo.pw.edu.pl/info/article/WUT0cda2d018b7e440f9be8c05e8c07aaa4/
- URN
urn:pw-repo:WUT0cda2d018b7e440f9be8c05e8c07aaa4
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or PerishOpening in a new tab system.