Comparison of fixed and living biological cells parameters investigated with digital holographic microscope

Patryk Kamil Nienałtowski , Maria Baczewska , Małgorzata Kujawińska

Abstract

The statistical analysis and comparison of biophysical parameters of living and fixed, mouse embryonic fibroblasts cells are presented. The parameters are calculated based on phase measurements performed by means of a digital, holographic microscope. The phases are retrieved from off-axis, image plane holograms, followed by custom image segmentation and statistical analysis of cells’ surface, phase volume and dry mass. The results indicated statistically significant differences between fixed and living cell parameters, which is an important message for setting methodology for further diagnosis based on quantitative phase (label-free) analysis.
Author Patryk Kamil Nienałtowski (FM)
Patryk Kamil Nienałtowski,,
- Faculty of Mechatronics
, Maria Baczewska (FM / IMPh)
Maria Baczewska,,
- The Institute of Micromechanics and Photonics
, Małgorzata Kujawińska (FM / IMPh)
Małgorzata Kujawińska,,
- The Institute of Micromechanics and Photonics
Journal seriesPhotonics Letters of Poland, ISSN 2080-2242
Issue year2020
Vol12
No1
Pages13-15
Publication size in sheets0.3
ASJC Classification2504 Electronic, Optical and Magnetic Materials
DOIDOI:10.4302/plp.v12i1.971
Languageen angielski
Score (nominal)40
Score sourcejournalList
ScoreMinisterial score = 40.0, 19-07-2020, ArticleFromJournal
Publication indicators Scopus Citations = 0; WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.348
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