Oligonucleotide-Based Electrochemical Biosensor for Hg2+ Using Methylene Blue as a Redox Indicator

Robert Ziółkowski , Marta Maria Jarczewska , Łukasz Górski , Elżbieta Malinowska

Abstract

Gold electrodesmodified with short-chain ss-DNA oligonucleotides were used for trace-level voltammetric determination ofmercury cation. External redox marker, methylene blue, was employed for these measurements. Due to the competition between Hg2+ and methylene blue, current signal decreases proportionally to the analyte concentration. It was shown that 5-SH-(CH2)6-(T)15−3 oligonucleotide, when employed as a receptor layer, induces the strongest electrochemical signal, among all tested DNA sequences. Electrochemical impedance spectroscopy measurements confirmed a specific interaction between the oligonucleotide receptor layer and Hg2+ ions. Proposed biosensor exhibited significant selectivity toward mercury ion, with strong discrimination of other common metal cations. The remarkably low detection limit of 4.63 nmol · L−1 for Hg2+ ions was calculated for proposed biosensor. The usefulness of developed biosensor was verified by its application for analysis of Hg2+ in aqueous samples.
Author Robert Ziółkowski (FC / IBC / DM)
Robert Ziółkowski,,
- Department Of Microbioanalytics
, Marta Maria Jarczewska (FC / IBC / DM)
Marta Maria Jarczewska,,
- Department Of Microbioanalytics
, Łukasz Górski (FC / IBC / DM)
Łukasz Górski,,
- Department Of Microbioanalytics
, Elżbieta Malinowska (FC / IBC / DM)
Elżbieta Malinowska,,
- Department Of Microbioanalytics
Journal seriesJournal of the Electrochemical Society, ISSN 0013-4651
Issue year2013
Vol160
No9
PagesB152-B155
Publication size in sheets0.5
ASJC Classification2505 Materials Chemistry; 1603 Electrochemistry; 2508 Surfaces, Coatings and Films; 3104 Condensed Matter Physics; 2105 Renewable Energy, Sustainability and the Environment; 2504 Electronic, Optical and Magnetic Materials
DOIDOI:10.1149/2.061309jes
URL http://jes.ecsdl.org/content/160/9/B152.short
Internal identifier5081
Languageen angielski
File
2013_Oligonucleotide-Based Electrochemical Biosensor.pdf 322.08 KB
Score (nominal)40
Score sourcejournalList
ScoreMinisterial score = 40.0, 01-02-2020, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 01-02-2020, ArticleFromJournal
Publication indicators WoS Citations = 3; Scopus Citations = 3; Scopus SNIP (Source Normalised Impact per Paper): 2013 = 1.309; WoS Impact Factor: 2013 = 2.859 (2) - 2013=2.903 (5)
Citation count*1 (2015-02-05)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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