Production of natural 2-phenylethanol: from biotransformation to purified product

Karolina Chreptowicz , Monika Wielechowska , Joanna Główczyk-Zubek , Ewelina Rybak , Jolanta Mierzejewska

Abstract

2-Phenylethanol (2-PE) is a volatile substance widely used in food, fragrance and cosmetics industries. Nowadays, most 2-PE originates from chemical synthesis. Since there is an increasing demand for natural products, we designed an alternative method of 2-PE production. The utilization of yeast biomass in the production of natural 2-PE is a promising alternative for both chemical synthesis and expensive plant extraction. Here, we investigated the JM2014 yeast strain, which we recently isolated from a fermented milk drink and classified as a species of Saccharomyces cerevisiae. To produce 2-PE, we used a 4 L batch culture of the JM2014 strain in a laboratory-scale bioreactor and, subsequently, developed a method of isolation and purification of 2-PE from the broth. Within 72 hours, the not-genetically modified JM2014 strain produced 3.60 g L-1 of 2-PE in the simple batch culture, which corresponds to an overall efficiency of the biotransformation step of 97.21%. Through the application of appropriately selected techniques and reagents, we extracted 2-PE that meets the criteria for a natural product with a 78% efficiency and a purity of >98% (GC). This is the first report documenting a complete methodology for 2- PE production, from biotransformation to purification of the final product.
Author Karolina Chreptowicz ZTiBSL
Karolina Chreptowicz,,
- Department Of Drug Technology And Biotechnology
, Monika Wielechowska ZTiBSL
Monika Wielechowska,,
- Department Of Drug Technology And Biotechnology
, Joanna Główczyk-Zubek ZTiBSL
Joanna Główczyk-Zubek,,
- Department Of Drug Technology And Biotechnology
, Ewelina Rybak ZTiBSL
Ewelina Rybak,,
- Department Of Drug Technology And Biotechnology
, Jolanta Mierzejewska ZTiBSL
Jolanta Mierzejewska,,
- Department Of Drug Technology And Biotechnology
Journal seriesFood and Bioproducts Processing, ISSN 0960-3085
Issue year2016
Vol100
NoPart A
Pages275-281
Publication size in sheets0.5
Keywords in English2-phenylethanol; biotransformation; extraction; L-phenylalanine; Saccharomyces cerevisiae; ethyl acetate
DOIDOI:10.1016/j.fbp.2016.07.011
URL http://www.sciencedirect.com/science/article/pii/S0960308516300839
Languageen angielski
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wdpb_publikacje_pliki_plik_publikacja_2970_org.pdf 1,009.95 KB
Score (nominal)35
ScoreMinisterial score = 35.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 1.97 (2) - 2016=2.966 (5)
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