Production of natural 2-phenylethanol: from biotransformation to purified product

Karolina Chreptowicz , Monika Wielechowska , Joanna Główczyk-Zubek , Ewelina Rybak , Jolanta Mierzejewska


2-Phenylethanol (2-PE) is a volatile substance widely used in food, fragrance and cosmetics industries. Nowadays, most 2-PE originates from chemical synthesis. Since there is an increasing demand for natural products, we designed an alternative method of 2-PE production. The utilization of yeast biomass in the production of natural 2-PE is a promising alternative for both chemical synthesis and expensive plant extraction. Here, we investigated the JM2014 yeast strain, which we recently isolated from a fermented milk drink and classified as a species of Saccharomyces cerevisiae. To produce 2-PE, we used a 4 L batch culture of the JM2014 strain in a laboratory-scale bioreactor and, subsequently, developed a method of isolation and purification of 2-PE from the broth. Within 72 hours, the not-genetically modified JM2014 strain produced 3.60 g L-1 of 2-PE in the simple batch culture, which corresponds to an overall efficiency of the biotransformation step of 97.21%. Through the application of appropriately selected techniques and reagents, we extracted 2-PE that meets the criteria for a natural product with a 78% efficiency and a purity of >98% (GC). This is the first report documenting a complete methodology for 2- PE production, from biotransformation to purification of the final product.
Author Karolina Chreptowicz (FC / IBC / DDTB)
Karolina Chreptowicz,,
- Department Of Drug Technology And Biotechnology
, Monika Wielechowska (FC / IBC / DDTB)
Monika Wielechowska,,
- Department Of Drug Technology And Biotechnology
, Joanna Główczyk-Zubek (FC / IBC / DDTB)
Joanna Główczyk-Zubek,,
- Department Of Drug Technology And Biotechnology
, Ewelina Rybak (FC / IBC / DDTB)
Ewelina Rybak,,
- Department Of Drug Technology And Biotechnology
, Jolanta Mierzejewska (FC / IBC / DDTB)
Jolanta Mierzejewska,,
- Department Of Drug Technology And Biotechnology
Journal seriesFood and Bioproducts Processing, ISSN 0960-3085
Issue year2016
NoPart A
Publication size in sheets0.5
Keywords in English2-phenylethanol; biotransformation; extraction; L-phenylalanine; Saccharomyces cerevisiae; ethyl acetate
ASJC Classification1500 General Chemical Engineering; 1303 Biochemistry; 1106 Food Science; 1305 Biotechnology
Languageen angielski
wdpb_publikacje_pliki_plik_publikacja_2970_org.pdf 1,009.95 KB
Score (nominal)35
Score sourcejournalList
ScoreMinisterial score = 35.0, 01-02-2020, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 01-02-2020, ArticleFromJournal
Publication indicators Scopus Citations = 19; WoS Citations = 16; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.191; WoS Impact Factor: 2016 = 1.97 (2) - 2016=2.966 (5)
Citation count*
Share Share

Get link to the record

* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Are you sure?