Enhanced chondrocytes proliferation in a wave-induced agitation system

Maciej Pilarek , Katarzyna Godlewska , Michał Wojasiński , Katarzyna Dąbkowska

Abstract

The applicability of a prototyped continuous wave-induced agitation system for intensification of anchorage-dependent CP5 chondrocytes proliferation outside and inside three-dimensional poly(lactic acid) (PLA) scaffolds has been discussed. Fibrous PLA-based constructs have been inoculated with CP5 cells and then maintained in two independent incubation systems: (i) nonagitated conditions and (ii) culture with wave-induced agitation. Significantly higher values of the glucose consumption rate have been noted for the system with the wave-induced agitation. The advantage of presented wave-induced agitation culture system has been confirmed by lower activity of lactate dehydrogenase (LDH) released from the cells in the samples of culture medium harvested from the agitated cultures, in contrast to rather high values of LDH activity measured for static conditions. Results of the proceeded experiments and their analysis clearly exhibited the feasibility of the culture system supported with continuously wave-induced agitation for robust proliferation of the CP5 chondrocytes on PLA-based structures. This work has been supported by the budget sources for The National Centre for Science, Poland, Grant no. DEC-2015/17/B/ST8/00631.
Author Maciej Pilarek (FCPE / DBBE)
Maciej Pilarek,,
- Department of Biotechnology and Bioprocess Engineering
, Katarzyna Godlewska (FC / CPCT)
Katarzyna Godlewska,,
- Chair Of Polymer Chemistry And Technology
, Michał Wojasiński (FCPE / DBBE)
Michał Wojasiński,,
- Department of Biotechnology and Bioprocess Engineering
, Katarzyna Dąbkowska (FCPE / DBBE)
Katarzyna Dąbkowska,,
- Department of Biotechnology and Bioprocess Engineering
Pages1127-1135
Publication size in sheets0.5
Book The 22nd Polish Conference of Chemical and Process Engineering: Proceedings, 2016, Faculty of Process and Environmental Engineering, ISBN 978-83-61997-75-7, 1584 p.
Proceedings OKIChiP 2016.pdf / No licence information (file archived - login or check accessibility on faculty)
Keywords in Englishwave-induced agitation, animal cell culture, CP5 chondrocytes, single-use bioreactor
Languageen angielski
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