Surface endothelialization of polyurethanes

Beata Aleksandra Butruk , Piotr Bąbik , Barbara Marczak , Tomasz Ciach

Abstract

One of the contemporary challenges for cardiovascular tissue engineers is to design hemocompatible biomaterials that promote neo-tissue formation. Despite many years of intensive research materials used to fabricate synthetic implants still cause clot formation in the bloodstream, which can have fatal consequences for the patient. Currently, the most promising strategy to fabricate non-thrombogenic materials is covering the implant surface with a layer of endothelial cells. Endothelial cells can be applied onto the synthetic implant before the procedure (in vitro endothelialization) or can be recruited from the bloodstream and the surrounding areas (in situ endothelialization). In the presented work, a method to modify polyurethane surface is proposed. The surface-modified materials are designed to selectively promote endothelial cell adhesion and proliferation. The modification is based on three-step process: surface activation, introduction of spacer and coupling of peptide to the spacer molecule. Colorimetric assays confirmed the successful incorporation of the proper functional groups after each modification step. As a modifying peptide we decided to use peptides containing REDV sequence, which has been proved to promote ECs adhesion and proliferation. In order to confirm the successful incorporation of peptides and visualize their surface distribution a set of materials with fluorescence stained peptides was prepared and analyzed with fluorescence microscopy. The number of HUVEC adhered to REDV-modified materials was significantly higher compared to the number of cells adhered to the non-modified polyurethane.
Author Beata Aleksandra Butruk (FCPE / DBBE)
Beata Aleksandra Butruk,,
- Department of Biotechnology and Bioprocess Engineering
, Piotr Bąbik (FCPE)
Piotr Bąbik,,
- Faculty of Chemical and Process Engineering
, Barbara Marczak (FCPE)
Barbara Marczak,,
- Faculty of Chemical and Process Engineering
, Tomasz Ciach (FCPE / DBBE)
Tomasz Ciach,,
- Department of Biotechnology and Bioprocess Engineering
Journal seriesProcedia Engineering, ISSN 1877-7058
Issue year2013
Vol59
Pages126-132
Publication size in sheets0.5
Conference3rd International Conference on Tissue Engineering, ICTE2013, 06-06-2013 - 08-06-2013, Leiria, Portugalia
Keywords in EnglishREDV peptide, surface modification, polyurethane, endothelial cell, blood coagulation, endothelialization, hemocompatibility
DOIDOI:10.1016/j.proeng.2013.05.101
URL http://www.sciencedirect.com/science/article/pii/S1877705813010151
Languageen angielski
File
ButrukB.,Bąbik.pdf 430.91 KB
Score (nominal)5
Score sourcejournalList
ScoreMinisterial score = 0.0, 02-04-2020, ArticleFromJournal
Ministerial score (2013-2016) = 5.0, 02-04-2020, ArticleFromJournal
Publication indicators Scopus Citations = 6; WoS Citations = 5; GS Citations = 8.0
Citation count*8 (2020-01-13)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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