Novel Perspectives on the Characterization of Species-Dependent Optical Signatures of Bacterial Colonies by Digital Holography

Igor Buzalewicz , Małgorzata Kujawińska , Wojciech Krauze , Halina Podbielska, prof.


The use of light diffraction for the microbiological diagnosis of bacterial colonies was a significant breakthrough with widespread implications for the food industry and clinical practice. We previously confirmed that optical sensors for bacterial colony light diffraction can be used for bacterial identification. This paper is focused on the novel perspectives of this method based on digital in-line holography (DIH), which is able to reconstruct the amplitude and phase properties of examined objects, as well as the amplitude and phase patterns of the optical field scattered/diffracted by the bacterial colony in any chosen observation plane behind the object from single digital hologram. Analysis of the amplitude and phase patterns inside a colony revealed its unique optical properties, which are associated with the internal structure and geometry of the bacterial colony. Moreover, on a computational level, it is possible to select the desired scattered/diffracted pattern within the entire observation volume that exhibits the largest amount of unique, differentiating bacterial features. These properties distinguish this method from the already proposed sensing techniques based on light diffraction/scattering of bacterial colonies. The reconstructed diffraction patterns have a similar spatial distribution as the recorded Fresnel patterns, previously applied for bacterial identification with over 98% accuracy, but they are characterized by both intensity and phase distributions. Our results using digital holography provide new optical discriminators of bacterial species revealed in one single step in form of new optical signatures of bacterial colonies: digital holograms, reconstructed amplitude and phase patterns, as well as diffraction patterns from all observation space, which exhibit species-dependent features. To the best of our knowledge, this is the first report on bacterial colony analysis via digital holography and our study represents an innovative approach to the subject.
Author Igor Buzalewicz
Igor Buzalewicz,,
, Małgorzata Kujawińska IMiF
Małgorzata Kujawińska,,
- The Institute of Micromechanics and Photonics
, Wojciech Krauze IMiF
Wojciech Krauze,,
- The Institute of Micromechanics and Photonics
, Halina Podbielska, prof.
Halina Podbielska, prof.,,
Journal seriesPlos One, ISSN 1932-6203
Issue year2016
Pages150449-1 - 150449-10
Publication size in sheets0.3
Keywords in PolishHolografia cyfrowa, kolonie bakterii, dyfrakcja, mikroskopia transmisyjna
Keywords in EnglishDigital holography, bacterial colonies, diffraction, transmission microscopy
Languageen angielski
Score (nominal)40
ScoreMinisterial score = 35.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.806 (2) - 2016=3.394 (5)
Citation count*5 (2018-02-17)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.