Stable pH responsive layer-by-layer assemblies of partially hydrolysed poly(2-ethyl-2-oxazoline) and poly(acrylic acid) for effective prevention of protein, cell and bacteria surface attachment

T. He , Dominik Jańczewski , S. Guo , S. M. Man , S. Jiang , W. S. Tan


Polyoxazolines have received increasing attention as low-fouling materials with good stability and ease of functional group incorporation. We investigated layer-by-layer (LbL) assembly of poly(2-ethyl-2-oxazoline) (PEOX) with poly(acrylic acid) (PAA) to incorporate PEOX into thin conformal coatings with controllable thicknesses ranging from the nano- to micron range. Partial hydrolysis of PEOX (to form PEOX-I) was used to introduce secondary amine groups that enable post-assembly multilayer stabilization by heat-induced crosslinking. While as-assembled multilayers dissolve in aqueous solutions at pH 5 and above, thermally crosslinked multilayers were stable against film loss and instead exhibit pH responsive swelling. The anti-fouling properties of crosslinked coatings were assessed by evaluating the resistance of PEOX-I containing multilayers to fouling by proteins, cells and bacteria. Our study of multilayers with thicknesses ranging from ∼12 nm to ∼1.5 m revealed thickness dependence of surface fouling resistance to BSA. Crosslinked multilayers of ∼220 nm were found to be highly effective in suppressing surface adsorption of bovine serum albumin (BSA), while thinner or thicker layers were increasingly susceptible to BSA adsorption. We further found that coatings of ∼220 nm and above were all highly effective at preventing surface attachment of fibroblasts, gram-positive (S. aureus) and gram-negative (E. coli) bacteria.
Author T. He - [A-Star, Institute of Materials Research and Engineering]
T. He,,
, Dominik Jańczewski (FC / LTP)
Dominik Jańczewski,,
- Laboratory Of Technological Processes
, S. Guo - [A-Star, Institute of Materials Research and Engineering]
S. Guo,,
, S. M. Man - [A-Star, Institute of Materials Research and Engineering]
S. M. Man,,
, S. Jiang - [Jilin University]
S. Jiang,,
, W. S. Tan - [A-Star, Institute of Materials Research and Engineering]
W. S. Tan,,
Journal seriesColloids and Surfaces B-Biointerfaces, ISSN 0927-7765, (A 35 pkt)
Issue year2018
Publication size in sheets0.5
Keywords in EnglishLayer-by-layer assembly Poly(2-ethyl-2-oxazoline) Polyoxazolines Anti-fouling Adhesion Protein adsorption Responsive
ASJC Classification1505 Colloid and Surface Chemistry; 1606 Physical and Theoretical Chemistry; 3110 Surfaces and Interfaces; 2700 General Medicine; 1305 Biotechnology
Abstract in Polishxxx
Languageen angielski
2018_CollSurfB_HeTao.pdf 2.3 MB
Score (nominal)35
ScoreMinisterial score = 35.0, 23-09-2019, ArticleFromJournal
Publication indicators Scopus Citations = 5; WoS Citations = 3; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.101; WoS Impact Factor: 2017 = 3.997 (2) - 2017=4.294 (5)
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