Effect of enzymatic hydrolysis on surface activity and surface rheology of type I collagen

Aleksandra Kezwoń , Ilona Chromińska , Tomasz Frączyk , Kamil Wojciechowski

Abstract

We describe the adsorption behaviour and rheological properties of a calf skin type I collagen, and of its hydrolysates obtained using a Clostridium histolyticum collagenase (CHC) under moderate conditions (pH 7, 37 °C). The effect of CHC concentration (2 × 10−9–2 × 10−6 M) and incubation time (35–85 min) was studied and optimised to achieve the highest decrease of surface tension and the highest dilational surface viscoelasticity of the adsorbed layers. SDS-PAGE electrophoresis and reverse-phase high performance liquid chromatography (RP-HPLC) were used to characterise the hydrolysis products. The results show that even simple modifications (heat treatment, pH change, partial hydrolysis) of collagen enhances its surface properties, especially in terms of surface dilational elasticity modulus. The use of low enzyme concentration (CHC-to-collagen molar ratio of 4 × 10−3) and short incubation time (<45 min) results in moderately hydrolysed products with the highest ability to lower surface tension (γ = 53.9 mN m−1) forming highly elastic adsorbed layers (surface dilational elasticity, E′ = 74.5 mN m−1).
Author Aleksandra Kezwoń ZMB
Aleksandra Kezwoń,,
- Department Of Microbioanalytics
, Ilona Chromińska ZMB
Ilona Chromińska,,
- Department Of Microbioanalytics
, Tomasz Frączyk
Tomasz Frączyk,,
-
, Kamil Wojciechowski ZMB
Kamil Wojciechowski,,
- Department Of Microbioanalytics
Journal seriesColloids and Surfaces B-Biointerfaces, ISSN 0927-7765
Issue year2016
Vol137
Pages60-69
Publication size in sheets0.5
Keywords in EnglishClostridium histolyticum; Calf skin collagen; Surface tension; Surface dilational rheology; Collagen hydrolysate
DOIDOI:10.1016/j.colsurfb.2015.05.017
URL http://www.sciencedirect.com.scopeesprx.elsevier.com/science/article/pii/S0927776515003148
Languageen angielski
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Score (nominal)35
ScoreMinisterial score = 35.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.887 (2) - 2016=4.295 (5)
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