The influence of selected ionic liquids on activity of chitin deacetylase

Izabela Aspras , Małgorzata Kamińska , Kamil Karzyński , Mateusz Kawka , Małgorzata Maria Jaworska

Abstract

Chitin deacetylase is the only known enzyme which is able to deacetylate N-acetyl-D-glucosamine units in chitin or chitosan chains. As chitin can hardly be dissolved in organic/inorganic solvents, new solvents are still searched. Ionic liquids are promising for that application and for homophase enzymatic deacetylation. The aim of the work was to investigate the influence of selected ionic liquids on activity of chitin deacetylase. It has been shown that [Amim] ionic liquids increase the activity of chitin deacetylase. The highest activity was observed for [Amim][Cl]. Ionic liquids with shorter (ethyl (C2)) and longer side chain (buthyl (C4)) only insignificantly influenced the activity of the enzyme. All tested ionic liquids with [Br] anion increased the activity of chitin deacetylase while the [Emim] and [Bmim] cation in combination with [Cl] anion inhibited the activity of the enzyme.
Author Izabela Aspras WIChiP
Izabela Aspras,,
- Faculty of Chemical and Process Engineering
, Małgorzata Kamińska WIChiP
Małgorzata Kamińska,,
- Faculty of Chemical and Process Engineering
, Kamil Karzyński WIChiP
Kamil Karzyński,,
- Faculty of Chemical and Process Engineering
, Mateusz Kawka WIChiP
Mateusz Kawka,,
- Faculty of Chemical and Process Engineering
, Małgorzata Maria Jaworska ZBIB
Małgorzata Maria Jaworska,,
- Department of Biotechnology and Bioprocess Engineering
Journal seriesChemical and Process Engineering , ISSN 0208-6425
Issue year2016
Vol37
No1
Pages77-82
Publication size in sheets0.3
Keywords in Englishchitin deacetylase, enzyme activity, imidasolium ionic liquids, pyridinium ionic liquids
DOIDOI:10.1515/cpe-2016-0008
URL https://www.degruyter.com/downloadpdf/j/cpe.2016.37.issue-1/cpe-2016-0008/cpe-2016-0008.pdf
Languageen angielski
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Aspras I. (i in.) - The influence of....pdf 215.19 KB
Score (nominal)15
ScoreMinisterial score = 15.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 15.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 0.971 (2) - 2016=0.925 (5)
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