Biological characterization of the modified poly(dimethylsiloxane) surfaces based on cell attachment and toxicity assays

Elżbieta Jastrzębska , Agnieszka Żuchowska , Sylwia Flis , Patrycja Sokolowska , Magdalena Bułka , Artur Dybko , Zbigniew Brzózka

Abstract

Poly(dimethylsiloxane) (PDMS) is a material applicable for tissue and biomedical engineering, especially based on microfluidic devices. PDMS is a material used in studies aimed at understanding cell behavior and analyzing the cell adhesion mechanism. In this work, biological characterization of the modified PDMS surfaces based on cell attachment and toxicity assays was performed. We studied Balb 3T3/c, HMEC-1, and HT-29 cell adhesion on poly(dimethylsiloxane) surfaces modified by different proteins, with and without pre-activation with plasma oxygen and UV irradiation. Additionally, we studied how changing of a base and a curing agent ratios influence cell proliferation. We observed that cell type has a high impact on cell adhesion, proliferation, as well as viability after drug exposure. It was tested that the carcinoma cells do not require a highly specific microenvironment for their proliferation. Cytotoxicity assays with celecoxib and oxaliplatin on the modified PDMS surfaces showed that normal cells, cultured on the modified PDMS, are more sensitive to drugs than cancer cells. Cell adhesion was also tested in the microfluidic systems made of the modified PDMS layers. Thanks to that, we studied how the surface area to volume ratio influences cell behavior. The results presented in this manuscript could be helpful for creation of proper culture conditions during in vitro tests as well as to understand cell response in different states of disease depending on drug exposure.
Author Elżbieta Jastrzębska (FC / IBC / DM)
Elżbieta Jastrzębska,,
- Department Of Microbioanalytics
, Agnieszka Żuchowska (FC / IBC / DM)
Agnieszka Żuchowska,,
- Department Of Microbioanalytics
, Sylwia Flis
Sylwia Flis,,
-
, Patrycja Sokolowska
Patrycja Sokolowska,,
-
, Magdalena Bułka (FC / IBC / DM)
Magdalena Bułka,,
- Department Of Microbioanalytics
, Artur Dybko (FC / IBC / DM)
Artur Dybko,,
- Department Of Microbioanalytics
, Zbigniew Brzózka (FC / IBC / DM)
Zbigniew Brzózka,,
- Department Of Microbioanalytics
Journal seriesBiomicrofluidics, ISSN 1932-1058, (A 30 pkt)
Issue year2018
Vol12
No044105
Pages044105_1-044105_14
Publication size in sheets2205.25
DOIDOI:10.1063/1.5035176
URL https://aip.scitation.org/doi/10.1063/1.5035176
Languageen angielski
File
wdpb_publikacje_pliki_plik_publikacja_3475_org.pdf 3.81 MB
Score (nominal)35
ScoreMinisterial score = 30.0, 06-08-2018, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 06-08-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.535 (2) - 2016=3.105 (5)
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