Tailoring polyelectrolyte architecture to promote cell growth and inhibit bacterial adhesion

S. Guo , M. Y. Kwek , Z. Q. Toh , D. Pranantyo , E.-T. Kang , X. J. Loh , X. Zhu , Dominik Jańczewski , K.G. Neoh

Abstract

An important challenge facing the application of implanted biomaterials for tissue engineering is the need to facilitate desirable tissue interactions with the implant while simultaneously inhibiting bacterial colonization, which can lead to implant-associated infection. In this study, we explore the relevance of the physical parameters of polyelectrolyte multilayers, such as surface charge, wettability, and stiffness, in tissue cell/surface and bacteria/surface interactions, and investigate the tuning of the multilayer architecture to differentially control such interactions. Polyions with different side-chain chemical structures were paired with polyethylenimine to assemble multilayers with parallel control over surface charge and wettability under controlled conditions. The multilayers can be successfully cross-linked to yield stiffer (the apparent Young’s modulus was increased more than three times its original value) and more stable films while maintaining parallel control over surface charge and wettability. The initial adhesion and proliferation of 3T3 fibroblast cells were found to be strongly affected by surface charge and wettability on the noncross-linked multilayers. On the other hand, these cells adhered and proliferated in a manner similar to those on the cross-linked multilayers (apparent Young’s modulus ∼2 MPa), regardless of surface charge and wettability. In contrast, Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) adhesion was primarily controlled by surface charge and wettability on both cross-linked and non-cross-linked multilayers. In both cases, negative charge and hydrophilicity inhibited their adhesion. Thus, a surface coating with a relatively high degree of stiffness from covalent cross-linking coupled with negative surface charge and high wettability can serve as an efficient strategy to enhance host cell growth while resisting bacterial colonization.
Author S. Guo - [National University of Singapore]
S. Guo,,
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, M. Y. Kwek - [National University of Singapore]
M. Y. Kwek,,
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, Z. Q. Toh - [National University of Singapore]
Z. Q. Toh,,
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, D. Pranantyo - [National University of Singapore]
D. Pranantyo,,
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, E.-T. Kang - [National University of Singapore]
E.-T. Kang,,
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, X. J. Loh - [A-Star, Institute of Materials Research and Engineering]
X. J. Loh,,
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, X. Zhu - [Zhejiang University]
X. Zhu,,
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, Dominik Jańczewski (FC / LTP)
Dominik Jańczewski,,
- Laboratory Of Technological Processes
, K.G. Neoh - [National University of Singapore]
K.G. Neoh,,
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Journal seriesACS Applied Materials & Interfaces, ISSN 1944-8244, (A 40 pkt)
Issue year2018
Vol10
Pages7882-7891
Publication size in sheets0.5
Keywords in Englishlayer-by-layer assembly, polyelectrolyte multilayer stiffness, surface charge, surface wettability, antimicrobial, cell adhesion and proliferation
ASJC Classification2500 General Materials Science
Abstract in Polishxxx
DOIDOI:10.1021/acsami.8b00666
URL www.acsami.org
Languageen angielski
File
2018_ACSApplMater_GuoSS.pdf 661.94 KB
Score (nominal)40
ScoreMinisterial score = 40.0, 30-04-2019, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 11-03-2019, ArticleFromJournal
Publication indicators Scopus Citations = 3; WoS Citations = 1; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.528; WoS Impact Factor: 2017 = 8.097 (2) - 2017=8.284 (5)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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