Continuous Recirculation of Microdroplets in a Closed Loop Tailored for Screening of Bacteria Cultures

Paweł R. Dębski , Karolina Skłodowska , Jacek A. Michalski , Piotr M. Korczyk , Mirosław Dolata , Sławomir Jakieła

Abstract

Emerging microfluidic technology has introduced new precision controls over reaction conditions. Owing to the small amount of reagents, microfluidics significantly lowers the cost of carrying a single reaction. Moreover, in two-phase systems, each part of a dispersed fluid can be treated as an independent chemical reactor with a volume from femtoliters to microliters, increasing the throughput. In this work, we propose a microfluidic device that provides continuous recirculation of droplets in a closed loop, maintaining low consumption of oil phase, no cross-contamination, stabilized temperature, a constant condition of gas exchange, dynamic feedback control on droplet volume, and a real-time optical characterization of bacterial growth in a droplet. The channels (tubing) and junction cubes are made of Teflon fluorinated ethylene propylene (FEP) to ensure non-wetting conditions and to prevent the formation of biofilm, which is particularly crucial for biological experiments. We show the design and operation of a novel microfluidic loop with the circular motion of microdroplet reactors monitored with optical sensors and precision temperature controls. We have employed the proposed system for long term monitoring of bacterial growth during the antibiotic chloramphenicol treatment. The proposed system can find applications in a broad field of biomedical diagnostics and therapy.
Author Paweł R. Dębski
Paweł R. Dębski,,
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, Karolina Skłodowska
Karolina Skłodowska ,,
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, Jacek A. Michalski (FCEMP / IC)
Jacek A. Michalski,,
- The Institute of Chemistry
, Piotr M. Korczyk
Piotr M. Korczyk,,
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, Mirosław Dolata
Mirosław Dolata ,,
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, Sławomir Jakieła
Sławomir Jakieła ,,
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Journal seriesMicromachines, ISSN 2072-666X, (A 30 pkt)
Issue year2018
Vol9(9)
No469
Pages1-11
Publication size in sheets0.5
Keywords in Englishmicrofluidic loop; bacteria cultures; screening; antibiotic treatment; Escherichia coli
ASJC Classification2208 Electrical and Electronic Engineering; 2210 Mechanical Engineering; 2207 Control and Systems Engineering
DOIDOI:10.3390/mi9090469
URL https://www.mdpi.com/2072-666X/9/9/469
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)30
ScoreMinisterial score = 30.0, 11-03-2019, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 11-03-2019, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2016 = 0.766; WoS Impact Factor: 2017 = 2.222 (2) - 2017=2.236 (5)
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