Continuous Recirculation of Microdroplets in a Closed Loop Tailored for Screening of Bacteria Cultures
Paweł R. Dębski , Karolina Skłodowska , Jacek A. Michalski , Piotr M. Korczyk , Mirosław Dolata , Sławomir Jakieła
AbstractEmerging microfluidic technology has introduced new precision controls over reaction conditions. Owing to the small amount of reagents, microfluidics significantly lowers the cost of carrying a single reaction. Moreover, in two-phase systems, each part of a dispersed fluid can be treated as an independent chemical reactor with a volume from femtoliters to microliters, increasing the throughput. In this work, we propose a microfluidic device that provides continuous recirculation of droplets in a closed loop, maintaining low consumption of oil phase, no cross-contamination, stabilized temperature, a constant condition of gas exchange, dynamic feedback control on droplet volume, and a real-time optical characterization of bacterial growth in a droplet. The channels (tubing) and junction cubes are made of Teflon fluorinated ethylene propylene (FEP) to ensure non-wetting conditions and to prevent the formation of biofilm, which is particularly crucial for biological experiments. We show the design and operation of a novel microfluidic loop with the circular motion of microdroplet reactors monitored with optical sensors and precision temperature controls. We have employed the proposed system for long term monitoring of bacterial growth during the antibiotic chloramphenicol treatment. The proposed system can find applications in a broad field of biomedical diagnostics and therapy.
|Journal series||Micromachines, ISSN 2072-666X, (A 30 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||microfluidic loop; bacteria cultures; screening; antibiotic treatment; Escherichia coli|
|ASJC Classification||; ;|
|License||Journal (articles only); published final; ; with publication|
|Score||= 30.0, 16-12-2019, ArticleFromJournal|
|Publication indicators||= 2; : 2016 = 0.766; : 2018 = 2.426 (2) - 2018=2.48 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.