A method of concentration estimation of trienes, tetraenes, and pentaenes in evening primrose oil

Bogdan Smyk , Piotr Zbigniew Wieczorek , Zadernowski Ryszard


There is no simple method available to estimate the concentration of conjugated fatty acids (CFA) with more than two double bonds in plant oils, because there are no commercially available complete sets of CFAs standards needed for such analysis. This paper presents such a method based on the absorption and fluorescence spectra of available CFAs standards and a computer program calculation. Using this method, the concentrations of the a-eleostearic acid (conjugated trienoic acid (ELA) 8.8 � 104 mol/L) and cis-parinaric acid (conjugated tetraenoic acid (PnA) 3.5 � 105 mol/L) in evening primrose oil were estimated. The accuracy of the calculation method is around 10%. Practical applications: This is the first report about the existence of CFAs in evening primrose oil. The described method estimates the total concentration of trienes, tetraenes, and pentaenes in evening primrose oils without using HPLC or other analytical methods. These CFAs are important from a health point of view. They are not synthesized by the human body. Therefore, plant oils containing these fatty acids (FA) are very valuable. Knowledge about the concentrations can be utilized to prepare a mixture of the plant oils with a favourable n-3 to n-6 FAs ratio and in addition containing CFAs.
Author Bogdan Smyk
Bogdan Smyk,,
, Piotr Zbigniew Wieczorek ISE
Piotr Zbigniew Wieczorek,,
- The Institute of Electronic Systems
, Zadernowski Ryszard
Zadernowski Ryszard,,
Journal seriesEuropean Journal of Lipid Science and Technology
Issue year2011
Publication size in sheets0.5
Keywords in English: Conjugated fatty acids / Evening primrose oil / Stripped oil / Tetraene / Triene
URL http://onlinelibrary.wiley.com/doi/10.1002/ejlt.201000418/pdf
Languageen angielski
Score (nominal)27
Citation count*4 (2018-02-20)
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